Asia

India’s Feluda Covid-19 test cheaper, faster alternative to RT-PCR, say scientists

NEW DELHI: More accurate than a rapid antigen test and almost as quick, India's CRISPR Feluda' Covid-19 test that changes colour on detection of the SARS-CoV-2 virus could be a cheaper, faster and simpler alternative to an RT-PCR diagnosis, say scientists.
Named after Satyajit Ray's famed detective, the Feluda test, which is priced at Rs 500 and can deliver a result in 45 minutes, is able to differentiate SARS-CoV-2 from other coronaviruses even if genetic variations between them are minute.
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Feluda test, developed by the New Delhi-based CSIR-Institute of Genomics and Integrative Biology (IGIB) and the TATA Group, received regulatory approvals last week from the Drug Controller General of India (DCGI) for commercial launch.
It meets high quality benchmarks with 96 per cent sensitivity and 98 per cent specificity for detecting the novel coronavirus, Debojyoti Chakraborty, a senior scientist at CSIR-IGIB and part of the team that developed the test, told PTI.
Sensitivity is defined as the ability of a test to correctly identify individuals with the disease, while specificity is the ability of the assay to accurately identify those without the disease.
Similar to a pregnancy strip test, Feluda changes colour if the virus is detected and doesn't need expensive machines for detection.
“The CRISPR technology uses a highly specific CAS9 protein to find and bind to the target Covid signature. This is then coupled with paper-strip chemistry to elicit a visual readout on a paper strip,” Chakraborty explained.
The test can help the country, with the world's second highest cases of Covid-19 at 61.45 lakh cases, ramp up testing of the disease quickly and economically, the researchers behind the test said in a statement.
Virologist Upasana Ray noted that the CRISPR based Covid-19 detection system is a cheaper option to RT-PCR tests, which cost over Rs 1,600. RAT and Feluda are in the same price bracket.
FELUDA, an acronym for the FNCAS9 Editor-Limited Uniform Detection Assay, uses an indigenously developed, cutting-edge CRISPR technology for detection of the genomic sequence of SARS-CoV-2 virus, the scientists said.
CRISPR is a gene editing technology and is used in correcting genetic defects and treating and preventing the spread of diseases.
The technology can detect specific sequences of DNA within a gene, and uses an enzyme functioning as molecular scissors to snip it.
According to Ray, Feluda is capable of detecting even low quantities of the genetic material of the novel coronavirus, based on very minute differences in their RNA – the genetic material of a virus
“Feluda is an alternative to the quantitative RT-PCR tests and is highly specific. It is capable of detecting low copy number nucleic acids (less viral RNA quantity) as well as single nucleotide variations,” Ray, a senior scientist at CSIR-IICB, Kolkata, told PTI.
She explained that the tests are so specific that they can distinguish SARS-CoV-2 infections from other coronaviruses such as the one that caused the 2002-03 SARS pandemic.
“It can distinguish between SARS-CoV-2 and SARS-CoV sequences which differ by a single nucleotide. Unlike RT-PCR which requires expensive machines, Feluda is simple and can be used in laboratories as well as outside with a quicker turnaround time,” she added.
In May, the US granted emergency-use approval for the world's first CRISPR-based test for Covid-19, developed by the Massachusetts Institute of Technology (MIT) and Harvard University.
The CRISPR Feluda test is the world's first diagnostic test to deploy a specially adapted Cas9Read More – Source

[contf]
[contfnew]

times of india

[contfnewc]
[contfnewc]

Related Articles

Asia

Joshua Wong and fellow activists plead guilty in Hong Kong protests trial

bbc– Hong Kong democracy activist Joshua Wong and two fellow campaigners are...

Asia

Works worth Rs 1,559 cr completed in Bihar out of PM s package of Rs 1.25 lakh cr: Congress

PATNA: Congress general secretary Randeep Surjewala on Tuesday claimed that works worth...

Asia

Ex-Trump fundraiser pleads guilty to illicit lobbying on 1MDB, China

WASHINGTON: A former top fundraiser for President Donald Trump pleaded guilty Tuesday...

Asia

Covid: Delhi more “open” than Mumbai

NEW DELHI: With Unlock 5.0 underway from October 15, more businesses and...